Comparison of the Golgi proteome of hepatocellular carcinoma with that of the adjacent non-tumor tissues / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 23-26, 2010.
Article
ي Zh
| WPRIM
| ID: wpr-247608
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To compare the Golgi proteome of hepatocellular carcinoma (HCC) with that of the adjacent non-tumor tissues.</p><p><b>METHODS</b>Hepatocellular carcinoma and adjacent non-tumor tissues were obtained from HCC patients. The protein expression maps in Golgi were obtained by two-dimensional gel electrophoresis (2-DE), and the differentially expressed protein spots were analyzed by PD-Quest software. Peptide mass fingerprint (PMF) of differential protein spots was obtained with MALD-TOT-MS.</p><p><b>RESULTS</b>According to 2-DE maps, the average numbers of protein spots were (1153+/-49) and (1086+/-37) in hepatocellular carcinoma and the adjacent non-tumor tissues. Compared to the adjacent non-tumor tissues, 27 proteins were upregulated, and 20 proteins were downregulated in HCC Golgi.</p><p><b>CONCLUSIONS</b>The Golgi proteome in HCC tissues is different from that in the adjacent non-tumor tissues, and the differential expression proteins are involved in energy metabolism, tumor metastasis, and cell cycle regulation.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
/
Electrophoresis, Gel, Two-Dimensional
/
Annexin A5
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Carcinoma, Hepatocellular
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Proteome
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Golgi Apparatus
/
Liver
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Liver Neoplasms
/
Metabolism
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Hepatology
السنة:
2010
نوع:
Article