Genotype and sequence analysis on G2 segments of hantavirus from HFRS patients in Hebei Province / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To know the genotype and subtype of hantavirus (HV) which infected persons in Hebei province.</p><p><b>METHODS</b>According to G2 coding region of 76-118 and R22 strains, specific type primers were designed to detect and identity the types of HV in HFRS patients' sera with RT-nested PCR. Nucleotides were assayed from partial products after purification and reclaim. Then, gene analysis was done with DNAStar package.</p><p><b>RESULTS</b>17 out of 69 positive serum specimens were successfully detected by RT-PCR and the detection rate was 24.64%, among which, <or= 7 days was 34.29%, 8-14 days was 19.23%, >or= 14 days were 0. 17 positive specimens were all belonged to SEO. The nucleotide homology of 9 typical specimens was 92.0%-100%. Between HeB7 and other 8 specimens was 92%-95%, and they belonged to different subtypes. When HeB7 compared with R22 strain, it was 97.7%. HeB7 and R22 belonged to S1 subtype. The 8 specimens except HeB7 was 95.7%-100% and they all belonged to S3 subtype. When compared with 76-118 strain, 9 specimens' nucleotide homology was only 70.3%-72.7%, belonged to different type.</p><p><b>CONCLUSION</b>SEO was the major type of HV from HFRS patients in Hebei province, S3 was the major subtype and S1 was also existed. In a certain area, the HV which belonged to the same type was correspondingly conservative, and had the characteristic of regional stability.</p>