Effect of Angelica sinensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma / 药学学报

ABSTRACT

<p><b>AIM</b>To study the effect of Angelica sinsensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma.</p><p><b>METHODS</b>Angelica sinensis polysaccharides(AP) were separated into AP-I, AP-II, AP-III and AP-IV by alcohol deposition with different concentration. The radioactivities of [3H]-TdR uptake by lymphocyte were used to determine the ability of lymphocyte. The bioactivity of IFN-gamma was measured by violet crystalline dying.</p><p><b>RESULTS</b>AP-IV was found to be composed of Ara and Glu in the ratio of 0.996.47, the molecular weight was estimated to be 5,600. AP-I and AP-II 100 mg.kg-1 i.p. were found to significantly augment mice splenocyte proliferation, release IFN-gamma and increase IFN-gamma bioactivity. 50 micrograms.mL-1 AP-I, AP-II and AP-III were shown to enhance the proliferative response of the mouse spleen lymphocytes in vitro.</p><p><b>CONCLUSION</b>AP-I and AP-II showed higher immunoactivity than AP-III, AP-IV had no effect.</p>