Effect of bortezomib on the drug sensitivity of imatinib resistant K562/G01 cells / 中华血液学杂志
Chinese Journal of Hematology
; (12): 392-395, 2011.
Article
ي Zh
| WPRIM
| ID: wpr-251943
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of bortezomib (BOR) on the drug sensitivity of imatinib-resistant chronic myeloid leukemia cell line K562/G01 cell and its mechanism.</p><p><b>METHODS</b>MTT assay was used to detect the inhibition effect of cell growth, flow cytometry to cell cycle, and real time-PCR to the expression of COX-2 and mdr1 mRNA.</p><p><b>RESULTS</b>Combination of 10 and 20 nmol/L BOR with imatinib could significantly enhance the sensitivity of K562/G01 to imatinib, the reverse factor was 1.83 and 2.72-fold respectively. Cell cycle arrested at G(2)/M phase could be observed by flow cytometry on BOR treatment. The over-expression of COX-2 and mdr1 could be down-regulated by BOR.</p><p><b>CONCLUSIONS</b>BOR can enhance the imatinib sensitivity of imatinib resistant K562/G01 cell. The mechanism may be related to cell cycle phase arrested at G2/M and down-regulation of COX-2 and mdr1 expression.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Piperazines
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Pyrazines
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Pyrimidines
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Benzamides
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Boronic Acids
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Cell Cycle
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Drug Resistance, Neoplasm
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ATP Binding Cassette Transporter, Subfamily B
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Hematology
السنة:
2011
نوع:
Article