Induction of petal-like structures from petals of Crocus sativus L. and the differentiation of style-stigma-like structures in vitro / 生物工程学报
Chinese Journal of Biotechnology
; (12): 638-640, 2002.
Article
ي Zh
| WPRIM
| ID: wpr-256147
المكتبة المسؤولة:
WPRO
ABSTRACT
Firstly the petal of Crocus sativus L was cultured on the medium that supplemented with different combinations of hormones. The petal-like structures(PLS) were induced on medium, but the induction rates were different in various medium. The highest induction rate of petal-like structures was obtained on the media that was supplemented with NAA (4 mg/L) and KT (8 mg/L). The petal-like structures were subcultured on another media when the structure was produced on the explants and proliferate groups. The later media was used for inducing style-stigma-like structures(SSLS). The induction rate of style-stigma-like-structures in the petal-like structures group is much higher than the rate in the preceding work, and the maximum of style-stigma-like structures produced per explant was 30. The best result of style-stigma-like structures was observed on the petal-like structure groups which came from the third treatment. The differentiation rate of style-stigma-like structures is stable in the subcultures of petal-like structures. The result revealed that the induction frequency of style-stigma-like structures formed on the petal-like structures is higher than that form on the petals of C. sativus L.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Cell Differentiation
/
Culture Media
/
Crocus
اللغة:
Zh
مجلة:
Chinese Journal of Biotechnology
السنة:
2002
نوع:
Article