Removal of oral Prevotella intermedia Endotoxin by octyl phenyl polyoxyethylene ether extraction method / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 725-729, 2012.
Article
ي Zh
| WPRIM
| ID: wpr-260200
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate an effective purification method for removing endotoxin from Prevotella intermedia.</p><p><b>METHODS</b>The main protein ingredients of bacteria prepared from ammonium sulfate precipitation were further treated with octyl phenyl polyoxyethylene ether (Triton X-114), and then processed at 4°C, 37°C and 25°C. The obtained aqueous phase after at least two more cycle repeated operations was assayed for endotoxin by Western blotting, LAL-clotting method, in vitro cell stimulation and in vivo animal experiments.</p><p><b>RESULTS</b>Western blotting and LAL-clotting method demonstrated that the reduction in endotoxin level was greater than 99.99% and recovery of the proteins after endotoxin removal was greater than 90% with Triton X-114 treatment for 3 cycles. The cytokines expression level was lower in both in vitro cell stimulation and in vivo animal experiments than in untreated group (P < 0.05).</p><p><b>CONCLUSIONS</b>The extraction method provides a new choice for endotoxin removal from large volumes of the oral Prevotella intermedia.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Polyethylene Glycols
/
Bacterial Proteins
/
Blood
/
Chemistry
/
Lipopolysaccharides
/
Interleukin-8
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Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Prevotella intermedia
المحددات:
Animals
/
Female
/
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Stomatology
السنة:
2012
نوع:
Article