Co-inhibition effect of 17-DMAG and oxaliplatin on proliferation and invasion of colorectal cancer cells / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 370-375, 2015.
Article
ي Zh
| WPRIM
| ID: wpr-260349
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of heat shock protein 90 (HSP90) inhibitor (17-DMAG) and oxaliplatin on the proliferation and invasion of colorectal cancer.</p><p><b>METHODS</b>After 17-DMAG, oxaliplatin and half-dose combination of 2 drugs processing colorectal cancer SW480 and HCT116 cell lines, CCK8 assay was applied to detect cell viability. RT-PCR and Western blot were used to detect the expression level of the apoptosis-related molecules. Transwell chemokine axis experiment and Western blot were employed to detect cell invasion ability and the expression level of tumor metastasis-associated protein.</p><p><b>RESULTS</b>The growth of SW480 and HCT116 cells was inhibited after the administration of 17-DMAG and oxaliplatin(P<0.05) in dose- and time-dependent manner. Processed by 17-DMAG 100 nmol/L, oxaliplatin 50 mg/L and half-dose combination of 2 drugs, transcription level of the apoptosis inhibitory gene (Bcl-2) in SW480 and HCT116 cells was decreased, the level of apoptosis promoting gene (Bax) transcription and protein PARP-1 spliceosome expression was increased, and the above trend was more obvious when using half-dose combination of 2 drugs. Transwell chemokine axis experiments showed the penetrating relative percentage and expression level of MMP9 and integrin β3 decreased, especially for half-dose combination of 2 drugs.</p><p><b>CONCLUSION</b>17-DMAG and oxaliplatin can co-inhibit the proliferation and invasion of colorectal cancer.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Organoplatinum Compounds
/
Colorectal Neoplasms
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Cell Survival
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Benzoquinones
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Apoptosis
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HCT116 Cells
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Lactams, Macrocyclic
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Cell Proliferation
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Neoplasm Invasiveness
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Antineoplastic Agents
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Gastrointestinal Surgery
السنة:
2015
نوع:
Article