Expression of B7 costimulator in eight human malignant hematological cell lines and its significance / 中华血液学杂志
Chinese Journal of Hematology
; (12): 345-348, 2002.
Article
ي En
| WPRIM
| ID: wpr-261417
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression of B7 co-stimulator in human malignant hematological cell lines and its significance.</p><p><b>METHODS</b>Amplified and purified plasmid DNA was transfected by liposome and detection of B7 gene expression by flow cytometry and RT-PCR.</p><p><b>RESULT</b>Eight human malignant hematological cell lines U937, K562, CEM, Daudi, GM, PEER, Jurkat, Raji express high level of HLA molecules, low or undetectable level of B7-1 gene, and high level of B7-2 gene. Expression level of B7-1 gene was enhanced after B7-1 transfection. T cell expressed high level of IL-2 mRNA after B7-1 transfection.</p><p><b>CONCLUSION</b>Multiple human malignant hematological cell lines express low or undetectable level of B7-1 gene, suggesting that B7-1 plays a critical role in tumor immunity.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
T-Lymphocytes
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Transfection
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Cell Line
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Interleukin-2
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B7-1 Antigen
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U937 Cells
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Allergy and Immunology
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Flow Cytometry
المحددات:
Humans
اللغة:
En
مجلة:
Chinese Journal of Hematology
السنة:
2002
نوع:
Article