DNA identification of Zijingpi's adulterant species Schisandra sphenanthera based on NCBI nucleotide database analysis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2534-2537, 2012.
Article
ي Zh
| WPRIM
| ID: wpr-263892
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To provide basis for quality control of Zijingpi, DNA identification was used based on NCBI nucleotide database analysis.</p><p><b>METHOD</b>Firstly, total DNA of Zijingpi was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR products was directly sequenced after purification. Finally, ITS sequence similarity and phylogenetic tree were used for identification.</p><p><b>RESULT</b>The ITS sequence information of the mainstream commercial drugs of Zijingpi was obtained.</p><p><b>CONCLUSION</b>It is firstly reported that the mainstream commercial drugs of Zijingpi was the bark of Schisandra sphenanthera.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phylogeny
/
Quality Control
/
Reference Standards
/
Drugs, Chinese Herbal
/
Molecular Sequence Data
/
Chemistry
/
Classification
/
Sequence Analysis, DNA
/
DNA, Plant
/
Databases, Nucleic Acid
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2012
نوع:
Article