Effects of Inhibiting Necroptosis on H9c2 Cardiomyocytes Injury Induced by Hypoxia/Reoxygenation / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 393-399, 2015.
Article
ي Zh
| WPRIM
| ID: wpr-266666
المكتبة المسؤولة:
WPRO
ABSTRACT
The aim of this study is to construct specific shRNA expressing plasmids, and to observe their effects on H9c2 cardiomyocytes injury induced by hypoxia/reoxygenation (H/R). RIPK1 and RIPK3 are the key kinases mediating the process of necroptosis. Using recombinant DNA technology, we inserted the synthetic shRNA into pSUPER vector to construct RIPK1-shRNA or RIPK3-shRNA plasmid respectively. We transfected H9c2 cardiomyocytes with the two shRNA plasmids respectively, before we treated them with H/R stimulation. Then, we measured the relevant genes and proteins by real-time PCR and Western blot. Meanwhile,we detected the markers of necroptosis and cardiomyocytes injury. The results showed that inhibition of ripk1 or ripk3 gene expression by its specific shRNA might protect the cardiomyocytes injury induced by H/R stimulation.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
/
Transfection
/
Cell Hypoxia
/
Gene Expression
/
Cell Line
/
Protein Serine-Threonine Kinases
/
Apoptosis
/
Myocytes, Cardiac
/
RNA, Small Interfering
/
Receptor-Interacting Protein Serine-Threonine Kinases
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Biomedical Engineering
السنة:
2015
نوع:
Article