FasL-cDNA transfected into mouse bone marrow cells ex vivo to prevent graft versus host disease / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 512-515, 2003.
Article
ي Zh
| WPRIM
| ID: wpr-278851
المكتبة المسؤولة:
WPRO
ABSTRACT
To explore the new approach to prevent graft versus host disease (GVHD) by purging ex vivo T lymphocytes of bone marrow graft through Fas-FasL way, FasL-cDNA was transfected into BALB/c mouse bon e marrow cells by liposome ex vivo. The transfected cells were cultured together with BAC (BALB/c x C57BL/6) mouse bone marrow graft. The mixing bone marrow graft was infused into BALB/c mouse recipients after 60Co-gamma irradiation. The mortality, manifestation and pathologic change of GVHD in recipient mice were observed. The CFU-S and Y chromosome from donor mice were detected. The results showed that compared with control group, the mortality in 60 days of the recipients in the experimental group decreased (20% vs 70%, P < 0.01) and the morbidity of GVHD lowered (40% vs 100%, P < 0.01). The CFU-S counts for all groups were at normal level on 20 days after transplantation. The Y chromosome from donor mice was discovered in 70% bone marrow nucleated cells of recipient mice survived over 2 months in the experimental group. It is concluded that mFasL-cDNA transfected mouse bone marrow cells prevent GVHD after culturing together with bone marrow graft, and accelerate hematopoietic reconstitution in recipient mice.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Bone Marrow Cells
/
Membrane Glycoproteins
/
Transfection
/
Genetic Therapy
/
Bone Marrow Transplantation
/
Bone Marrow Purging
/
Fas Ligand Protein
/
Genetics
/
Graft vs Host Disease
/
Metabolism
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2003
نوع:
Article