Immune response induced by HIV DNA vaccine combined with recombinant adeno-associated virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 251-254, 2004.
Article
ي Zh
| WPRIM
| ID: wpr-279562
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>HIV-1 DNA vaccine and recombinant adeno-associated virus (rAAV) expressing gagV3 gene of HIV-1 subtype B were constructed and BALB/c mice were immunized by vaccination regimen consisting of consecutive priming with DNA vaccine and boosting with rAAV vaccine; the CTL and antibody response were detected and compared with those induced by DNA vaccine or rAAV vaccine separately.</p><p><b>METHODS</b>HIV-1 subtype B gagV3 gene was inserted into the polyclonal site of plasmid pCI-neo, DNA vaccine pCI-gagV3 was thereby constructed; pCI-gagV3 was transfected into p815 cells, G-418-resistant cells were obtained through screening transfected cells with G418, the expression of HIV-1 antigen in G-418-resistant cells was detected by EIA; BALB/c mice were immunized with pCI-gagV3 and the immune response was tested; BALB/c mouse immunized with pCI-gagV3 and combined with rAAV expressing the same gagV3 genes were tested for antibody level in sera by EIA method and cytotoxicity response by LDH method.</p><p><b>RESULTS</b>pCI-gagV3 could express HIV-1 gene in p815 cells; pCI-gagV3 could induce HIV-1 specific humoral and cell-mediated immune response in BALB/c mice. The HIV-1 specific antibody level was 1/20; when the ratio of effector cells target cells was 501, the average specific cytotoxicity was 41.7%; there was no evident increase in the antibody level induced by pCI-gagV3 combined with rAAV, but there was increase in CTL response, the average specific cytotoxicity was 61.3% when effector cells target cells ratio was 501.</p><p><b>CONCLUSION</b>HIV-1 specific cytotoxicity in BALB/c mice can be increased by immunization of BALB/c mice with DNA vaccine combined with rAAV vaccine.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
/
Recombination, Genetic
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Blood
/
Transfection
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T-Lymphocytes, Cytotoxic
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Gene Products, gag
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HIV-1
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AIDS Vaccines
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Dependovirus
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Vaccines, DNA
المحددات:
Animals
اللغة:
Zh
مجلة:
Chinese Journal of Experimental and Clinical Virology
السنة:
2004
نوع:
Article