Application of reverse transcription-multiplex nested PCR to detect MLL rearrangement in AML-M4/M5 / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 444-446, 2005.
Article
ي Zh
| WPRIM
| ID: wpr-280030
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the value of reverse transcription-multiplex nested PCR in detecting MLL rearrangement in lzAML-M4/M5.</p><p><b>METHODS</b>Bone marrow chromosome preparation was made using direct method or short-term culture. Karyotypic analysis was carried out by R-banding technique. Five common MLL fusion genes and MLL partial tandem duplication in 40 AML cases, including 12 M4 and 28 M5 were detected by reverse transcription(RT)-multiplex nested PCR.</p><p><b>RESULTS</b>R-banding karyotypic analysis revealed 11q23 translocation including t(6;11)(q27;q23), t(9;11)(p21;q23), t(11;17)(q23;q21) and t(11;19)(q23;p13.1) in 7 cases. MLL rearrangements consisting of MLL/AF6 (1 case), MLL/AF9 (1 case), MLL/AF17 (2 cases), MLL/ELL (2 cases) and MLL partial tandem duplication(2 cases) were detected in 8 cases by RT-multiplex nested PCR. Among 8 cases with MLL rearrangement, 6 were chromosome translocation, 2 were MLL partial tandem duplication.</p><p><b>CONCLUSION</b>RT-multiplex nested PCR is a powerful technique in the detection of MLL rearrangement for tentativelly diagnosed AML-M4/M5.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Translocation, Genetic
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Leukemia, Myelomonocytic, Acute
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Reproducibility of Results
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Reverse Transcriptase Polymerase Chain Reaction
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Myeloid-Lymphoid Leukemia Protein
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Genetics
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Karyotyping
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Methods
المحددات:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
اللغة:
Zh
مجلة:
Chinese Journal of Medical Genetics
السنة:
2005
نوع:
Article