Association of TNFA polymorphisms with the outcomes of HBV infection / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 406-410, 2005.
Article
ي Zh
| WPRIM
| ID: wpr-280040
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether the TNFA promoter single nucleotide polymorphisms (SNPs) are associated with the outcomes of hepatitis B virus(HBV) infection in Chinese Han population.</p><p><b>METHODS</b>One hundred and forty-eight self-limited HBV infection subjects and 207 chronic hepatitis B patients were recruited. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequence specific primer-PCR(PCR-SSP) were used to detect the SNPs of five sites in TNFA promoter (-238G/A, -308G/A, -857C/T, -863C/A, -1031T/C). The frequency distributions of genotypes and haplotypes in different groups were analyzed by EPI and EH programs.</p><p><b>RESULTS</b>The frequencies of -238GG genotype in chronic hepatitis B patients were significantly higher than that in self-limited infection subjects (P=0.02). The frequencies of -857TT genotype in chronic hepatitis B patients were clearly lower than that in self-limited infection subjects (P=0.02). Haplotypic frequencies of GGCCT (-238/-308/-857/-863/-1031) in chronic hepatitis B patients was significantly lower than that in self-limited infection subjects (P=0.03), and the frequencies of haplotype GGCAT or GGTAT in chronic hepatitis B patients were clearly higher than those in self-limited infection subjects (P=0.0001; P=0.004).</p><p><b>CONCLUSION</b>TNFA promoter polymorphisms are important host genetic factors affecting the outcomes of HBV infection.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Haplotypes
/
Polymorphism, Restriction Fragment Length
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Polymerase Chain Reaction
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Tumor Necrosis Factor-alpha
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Genetic Predisposition to Disease
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Polymorphism, Single Nucleotide
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Gene Frequency
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Genetics
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Genotype
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Hepatitis B
المحددات:
Adult
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Female
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Humans
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Male
اللغة:
Zh
مجلة:
Chinese Journal of Medical Genetics
السنة:
2005
نوع:
Article