Tissue culture and polyploidy induction of Morinda officinalis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2325-2328, 2011.
Article
ي Zh
| WPRIM
| ID: wpr-283202
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish an effective protocol for plant generation and induce polyploidy of Morinda offcinalis.</p><p><b>METHOD</b>Callus was induced from immature embryo of M. officinalis and polyploidy was inducted by using colchicine treatment method. Chromosome was detected by flow cytometry.</p><p><b>RESULT AND CONCLUSION</b>The highest induction rate of polyploidy was 18.40%, which was obtained with 500 mg x L(-1) colchicine treatment for 5 days. Roots of polyploid were bigger than diploid. Advantages of using immature embryo as explants are easy for sterilization, higher rate of callus induction and low degree dedifferentiation. The induced polyploidy of M. officinalis may have a value for spread of cultivation.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Polyploidy
/
Chromosomes, Plant
/
Morinda
/
Tissue Culture Techniques
/
Genetics
/
Methods
نوع الدراسة:
Guideline
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2011
نوع:
Article