Preliminary study on effect of Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces on intestinal flora of mice / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3053-3058, 2015.
Article
ي Zh
| WPRIM
| ID: wpr-284800
المكتبة المسؤولة:
WPRO
ABSTRACT
This study aims to analyze and compare the effect of cell wall-broken decoction pieces, conventional decoction pieces and conventional powder of Rhodiolae Crenulatae Radix et Rhizoma on the intestinal flora of normal mice. The conventional bacterial culture and PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) were adopted for the mice after the oral administration for 14 days. According to the bacterial culture results, the 1/8 dose cell wall-broken decoction pieces group showed fewer Enterococcus and Escherichia coli bacillus but more Lactobacillus and Bifidobacterium than the conventional decoction pieces group and the traditional powder group (P <0.05). Meanwhile, on the basis of the PCR-DGGE results, the 1/8 dose cell wall-broken decoction pieces group revealed the highest Shannon-Wiener index (H) and species richness (S) among the seven groups, with extremely significant differences compared with the normal group (P <0.01), significant differences compared with the conventional decoction pieces group and the conventional powder group (P <0.05) and a high intra-group similarity. In conclusion, the long-term intake of 1/8 dose Rhodiolae Crenulatae Radix et Rhizoma cell wall-broken decoction pieces showed a certain effect in regulating intestinal tract by promoting the growth of Lactobacillus and Bifidobacterium. Furthermore, the intestinal flora community will become more stable.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Bifidobacterium
/
Cell Wall
/
Polymerase Chain Reaction
/
Rhodiola
/
Rhizome
/
Denaturing Gradient Gel Electrophoresis
/
Genetics
/
Intestines
/
Lactobacillus
/
Mice, Inbred C57BL
المحددات:
Animals
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2015
نوع:
Article