A preliminary in vitro study on the activation of polymorphonuclear cells and vascular endothelial cells co-cultured with and stimulated by lipopolysaccharide / 中华烧伤杂志
Chinese Journal of Burns
; (6): 78-80, 2002.
Article
ي Zh
| WPRIM
| ID: wpr-289185
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the activation of polymorphonuclear cells (PMNs) and vascular endothelial cells co-cultured with and stimulated by lipopolysaccharide.</p><p><b>METHODS</b>PMNs in concentration of 2 x 10(6)/ml isolated from healthy volunteers by Percoll gradient were added to monolayer of ECV-304 cells grown to confluency, then different groups were prepared according to final concentration of lipopolysaccharide. The morphological change was observed under invert microscope. The changes in TNFalpha and IL-6 levels of the supernatant of the cultured cells were determined at 4, 8, 12 and 24 hours after culturation.</p><p><b>RESULTS</b>The TNFalpha production of cultured pure ECV-304 exhibited no remarkable change when stimulated by different concentrations of LPS. But the TNFalpha production of the ECV-304 increased significantly when co-cultured with PMNs at 4 hr and stimulated by LPS in concentration of 10 micro g/ml, and increased at 8 hours and lasted up to 24 hours of culturation in higher levels (P < 0.05). The IL-6 production of cultured pure ECV-304 increased obviously along with the increase of LPS concentration, and it showed no change when PMNs co-cultured with ECV-304. While the IL-6 level in the supernatant of co-cultured ECV-304 with PMNs increased sharply when stimulated by both low (100 ng/ml) and high (1 micro g/ml) concentrations of LPS and maintained at high levels up to 24 hours of culturation. The higher the concentration of LPS was, the quicker the IL-6 level increased.</p><p><b>CONCLUSION</b>Co-cultured PMNs and endothelial cells could be activated and activation state could be maitained by low concentrations of LPS.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Time Factors
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Endothelium, Vascular
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Cell Line
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Cells, Cultured
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Lipopolysaccharides
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Coculture Techniques
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Cell Biology
المحددات:
Adult
/
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Burns
السنة:
2002
نوع:
Article