The kinetics of hematopoietic cell chimerism in the early period after non-myeloablative transplantation and its clinical implications / 中华血液学杂志
Chinese Journal of Hematology
; (12): 466-469, 2004.
Article
ي Zh
| WPRIM
| ID: wpr-291396
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the kinetics of hematopoietic cell chimerism in the early period after non-myeloablative stem cell transplantation (NAST) and to investigate the correlation between molecular and hematologic assessment of engraftment or rejection.</p><p><b>METHOD</b>Short tandem repeat-polymerase chain reaction (STR-PCR) analysis of chimerism status was carried out in 6 patients who received NAST from HLA-matched sibling donors.</p><p><b>RESULTS</b>In 5/6 patients, the peripheral blood samples collected on the first day after allograft infusion displayed the presence of mixed chimerism. STR-PCR analysis revealed a gradual increase of the donor-specific allelic signal which became dominant over the recipient-specific allele by day +7. On day +14, hematologic chimerisms were completely donor origin. Their molecular engraftments (ME) were detected at a median time of 6 days, preceding hematologic engraftment by a median of 5 days (P > 0.05). But the sixth patient showed more than 50% host residual cells on day +7 and had no signs of ME on day +14.</p><p><b>CONCLUSION</b>It suggested that molecular monitoring of the early dynamics of chimerism after NAST could be useful in predicting engraftment, or rejection. If the engraftment was less than 50% on day +7 and failed to get ME on day +14, the graft rejection would occur.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Transplantation, Homologous
/
Kinetics
/
Polymerase Chain Reaction
/
Transplantation Chimera
/
Hematopoietic Stem Cell Transplantation
/
Tandem Repeat Sequences
/
Diagnosis
/
Graft Rejection
/
Graft vs Host Disease
/
Hematopoiesis
نوع الدراسة:
Diagnostic_studies
المحددات:
Adult
/
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Hematology
السنة:
2004
نوع:
Article