Optinization of rapid propagation technique and induction and identification of autotetraploid of Polygonum multiflorum / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1467-1470, 2013.
Article
ي Zh
| WPRIM
| ID: wpr-294088
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish and optimize the rapid propagation system of Polygonum multiflorum, as well as explore method for induction and identification of autotetraploid.</p><p><b>METHOD</b>Propagation medium was optimized by orthogonal test. The buds were immersed in colchicine solution with different concentrations for different time to select induction conditions for autotetraploid of P. multiflorum.</p><p><b>RESULT</b>The most appropriate propagation medium was MS medium supplemented with 1.0 mg x L(-1) 6-BA, 0.3 mg x L(-1) NAA, and 0.4 mg x L(-1) PP333. That the buds were soaked in 0.2% colchicine solution for 30 h, or soaked in 0.3% colchicine solution for 18 h, was optimal condition to induce autopolyploid of P. multiflorum with induction rate as high as 16.7%.</p><p><b>CONCLUSION</b>Rapid propagation of P. multiflorum could be achieved by tissue culture. Furthermore, colchicine was an effective inducer of polyploidy, and 25 tetraploid lines were obtained through chromosome identification. The experiment laid a foundation for the wild resource conservation, superior varieties breeding of P. multiflorum.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Culture Media
/
Polygonum
/
Chromosomes, Plant
/
Tissue Culture Techniques
/
Tetraploidy
/
Genetics
/
Metabolism
/
Methods
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2013
نوع:
Article