cDNA array in the establishment of a gene expression profile associated with differentiation inducing the glioma cells / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 222-225, 2002.
Article
ي Zh
| WPRIM
| ID: wpr-301969
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.</p><p><b>METHOD</b>The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.</p><p><b>RESULTS</b>Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.</p><p><b>CONCLUSION</b>A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
/
DNA, Neoplasm
/
RNA, Neoplasm
/
Gene Expression
/
Cell Differentiation
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Genetics
/
Glioma
/
Metabolism
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Oncology
السنة:
2002
نوع:
Article