Platinum tetrachloride coupled PEG-PEI as drug carrier and gene delivery vector / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To construct a drug carrier and gene vector PEG-PEI-Pt.</p><p><b>METHODS</b>Polyethyleneglycol (PEG) was coupled to polyethylenimine (PEI 600) and platinum tetrachloride; PEG-PEI-Pt complex was formed in ethanol. The complex was characterized by XRD, UV-VIS and FT-IR and the DNA condensation was tested by electrophoretic mobility shift assay. The cell viability was evaluated by MTT assay in Hela, B16, A293 and COS-7 cells and in vitro transfection efficiency was measured in A293 and B16 cells.</p><p><b>RESULT</b>The structure of PEG-PEI-Pt was characterized by XRD, UV-VIS and FT-IR. PEG-PEI-Pt complex was able to bind DNA at N/P weight ratio of 0.4:1; the complex showed cytotoxicity on Hela and B16 cells. The complex had higher transfection efficiency in A293 and B16 cells than PEI 600.</p><p><b>CONCLUSION</b>A novel drug carrier and gene vector PEG-PEI-Pt was constructed successfully.</p>