Application of detecting gene rearrangement in diagnosing and typing of primary non-Hodgkin's lymphoma in nasal cavity / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
; (12): 932-935, 2006.
Article
ي Zh
| WPRIM
| ID: wpr-315561
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the significance of immunoglobulin heavy chain (IgH) gene rearrangement for B-cell lymphoma and T-cell receptor (TCR) gene rearrangement for T-cell lymphoma and NK/T-cell lymphoma in diagnosing and typing of primary non-Hodgkin's lymphoma (NHL) in nasal cavity.</p><p><b>METHODS</b>Semi-nested polymerase chain reaction ( PCR) with two pairs of primers was used to detect monoclonal IgH gene rearrangement in paraffin-embedded tissues from 11 patients with B-cell lymphoma, and one-stepped PCR with two pairs of primers was used to detect T-cell receptor gene rearrangement from 23 patients with NK/T-cell lymphoma and 20 patients with T-cell lymphoma. Ten patients with nasal polyp were detected with all the primers by PCR respectively.</p><p><b>RESULTS</b>Among the 54 patients with an evaluable PCR results, 10 of 11 (90. 9% ) B-cell lymphomas were positive for monoclonal IgH gene rearrangement, 17 of 20 (85. 0% ) T-cell lymphomas and 10 of 23 (43. 5% ) NK/T-cell lymphomas were positive for monoclonal TCR gene rearrangement. Ten patients with nasal polyp were negative for all detection.</p><p><b>CONCLUSIONS</b>Detecting gene rearrangement was an efficient method in auxiliary diagnosing and typing of primary NHL in nasal cavity; Using semi-nested PCR or one-stepped PCR with two pairs of primers can enhance the positive rate of gene rearrangement detection.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pathology
/
Lymphoma, Non-Hodgkin
/
Gene Rearrangement, B-Lymphocyte, Heavy Chain
/
Gene Rearrangement, T-Lymphocyte
/
Nose Neoplasms
/
Diagnosis
/
Nasal Cavity
نوع الدراسة:
Diagnostic_studies
المحددات:
Adolescent
/
Adult
/
Aged
/
Child
/
Female
/
Humans
/
Male
اللغة:
Zh
مجلة:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
السنة:
2006
نوع:
Article