Determination of astragulin in Thesium chinese by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a determination method of astragulin in Thesium chinese.</p><p><b>METHOD</b>RP-HPLC analytical method was established using a Polaris C18 column and acetonitrile-water (23:77) as the mobile phase, with a flow rate of 10 mL x min(-1), detected at 346 nm. The method of sample is refluxing exation by 50% alcohol for 2 times.</p><p><b>RESULT</b>The content of astragulin was from 0. 120% to 0. 155%, in different groups of T. chinese collected from the same location.</p><p><b>CONCLUSION</b>The method was validated to show convenient and reliable.</p>