Screening and application of prokaryotic enhancer-like sequence 3A / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 175-177, 2010.
Article
ي Zh
| WPRIM
| ID: wpr-316932
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen enhancer-like sequences from Escherichia coli strain C600 genome, to construct an expression vector harboring prokaryotic enhancer-like sequence and study the effect of interferon gene expression.</p><p><b>METHODS</b>Enhancer-like element from Escherichia coli strain C600 genome was obtained by using the chloramphenicol acetyl-transferase (CAT) gene as reporter gene. An expression vector harboring prokaryotic enhancer-like sequence from Escherichia coli strain C600 was constructed. Interferon was expressed and assayed.</p><p><b>RESULTS</b>An enhancer-like sequences with distance and orientation independence property were screened and named 3A. Quantification test showed that the direct and reverse orientation of 3A could increase the activity of beta-galactosidase with 7.11 and 2.93 times. The enhancing activity of the element was on transcription level. An expression vector harboring the prokaryotic enhancer-like sequence 3P3 which was enhancing function region of sequence 3A was constructed. Using this vector the antiviral activity of interferon alpha-2b was increased by 3.7 times in comparison with the original expression plasmid.</p><p><b>CONCLUSION</b>3A enhancer-like sequence was screened from Escherichia coli strain C600 genome. Interferon gene was highly expressed by using an expression vector harboring enhancer-like sequences.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Prokaryotic Cells
/
Sequence Homology, Nucleic Acid
/
Gene Expression
/
Chemistry
/
Enhancer Elements, Genetic
/
Interferons
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Beta-Galactosidase
/
Genes, Reporter
/
Escherichia coli
/
Genetic Vectors
نوع الدراسة:
Diagnostic_studies
/
Screening_studies
اللغة:
Zh
مجلة:
Chinese Journal of Experimental and Clinical Virology
السنة:
2010
نوع:
Article