5-lipoxygenase is involved in rotenone-induced injury in PC12 cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 150-155, 2011.
Article
ي Zh
| WPRIM
| ID: wpr-319817
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether 5-lipoxygenase (5-LOX) is involved in rotenone-induced injury in PC12 cells, which is a cell model of Parkinson disease.</p><p><b>METHODS</b>After rotenone treatment for various durations, cell viability was determined by colorimetric MTT reduction assay, and 5-LOX translocation was detected by immunocytochemistry. The effect of 5-LOX inhibitor zileuton was also investigated.</p><p><b>RESULT</b>Rotenone (0.3-30 μmol/L) induced PC12 cell injury, and zileuton (3-100 μmol/L) attenuated this injury. Rotenone also time-and concentration-dependently induced 5-LOX translocation into the nuclear envelope, and zileuton (1-30 μmo/L) significantly inhibited rotenone-induced 5-LOX translocation.</p><p><b>CONCLUSION</b>5-LOX is involved in rotenone-induced injury in PC12 cells, and 5-LOX inhibitor zileuton can reduce rotenone-induced 5-LOX activation and cell injury.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Physiology
/
Rotenone
/
Arachidonate 5-Lipoxygenase
/
Cell Survival
/
PC12 Cells
/
Lipoxygenase Inhibitors
/
Hydroxyurea
/
Metabolism
المحددات:
Animals
اللغة:
Zh
مجلة:
Journal of Zhejiang University. Medical sciences
السنة:
2011
نوع:
Article