Differentiation of human umbilical cord derived mesenchymal stem cells into low immunogenic and functional hepatocyte-like cells in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 190-194, 2010.
Article
ي Zh
| WPRIM
| ID: wpr-322802
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the biological function of hepatocyte-like cells derived from mesenchymal stem cells that isolated from human umbilical cord UC-MSCs in vitro, and to detect the changes in the immunogenicity of the differentiated hepatocyte-like cells (DHC).</p><p><b>METHODS</b>Transdifferentiation of UC-MSCs into hepatic lineage in vitro was induced in modified two-step induction medium. The expressions of hepatic specific markers were detected by RT-PCR analysis and immunofluorescence staining at different time points after induction. The levels of albumin and urea in the supernatants of cultures were measured by enzyme-linked immunosorbent assay. Furthermore, the immunosuppressive property of DHC was detected by one-way mixed lymphocyte culture.</p><p><b>RESULTS</b>The mRNA and proteins of alpha fetoprotein (AFP), albumin (ALB),and cytokeratin-19 (CK-19) were expressed in naive UC-MSCs at low levels. DHC highly expressed hepatic markers AFP, ALB, CK-19, and tryptophan 2, 3-dioxygenase 14 and 28 days after hepatic differentiation and were accompanied by an increased production of ALB and urea in supernatant in a time-dependent manner. DHC did not express human leukocyte antigen DR antigen and significantly decreased the lymphocyte proliferation.</p><p><b>CONCLUSION</b>UC-MSCs are able to differentiate into functional hepatocyte-like cells in vitro, while the immunogenicity of DHC remains low.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Umbilical Cord
/
Cells, Cultured
/
Hepatocytes
/
Cell Biology
/
Allergy and Immunology
/
Cell Transdifferentiation
/
Mesenchymal Stem Cells
المحددات:
Humans
اللغة:
Zh
مجلة:
Acta Academiae Medicinae Sinicae
السنة:
2010
نوع:
Article