Analysis of influence factors on G-CSF-mobilized hematopoietic stem cells of 191 healthy donors in a mono center / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 169-172, 2013.
Article
ي Zh
| WPRIM
| ID: wpr-325191
المكتبة المسؤولة:
WPRO
ABSTRACT
This study was aimed to investigate the factors influencing mobilization efficiency of peripheral hematopoietic stem cells with granulocyte colony stimulating factor (G-CSF) and their impact on healthy donors. 181 donors were mobilized subcutaneously with G-CSF at 5 - 10 µg/(kg·d), and 10 donors were mobilized with G-CSF at 3.3 - 4.9 µg/(kg·d), once 12 h, for 4 - 5 d. Peripheral blood mononuclear cell (MNC) and CD34(+) cell counts were analyzed by flow cytometry. Mobilization-related side effects were also monitored. The results showed that white blood cell counts increased by 6 times averaged after mobilization (P < 0.01). The platelet count obviously decreased (P < 0.01), while the hemoglobin level did not show significant difference. No significant differences were observed in MNC and CD34(+) cell counts between those subjects harvested on the 4th and 5th day after mobilization. Male donors were superior to female ones in cell harvest (P < 0.01). Donor body weight played positive role in cell yield, while impact of age on harvest was not remarkable. Neither MNC nor CD34(+) cell count showed a linear relationship with G-CSF dose. Only slight side effects were observed on the donors in this study. It is concluded that mobilization with G-CSF is sufficient in healthy donors without remarkable side effects.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Tissue Donors
/
Factor Analysis, Statistical
/
Granulocyte Colony-Stimulating Factor
/
Hematopoietic Stem Cell Mobilization
/
Peripheral Blood Stem Cell Transplantation
/
Methods
المحددات:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2013
نوع:
Article