A method of detect the virus which caused diarrhea / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Building a method which can examines virus pathogenic in gastroenteritis excrement specimen.</p><p><b>METHODS</b>Choosing six positive specimens which tested in our laboratory, include adenovirus, calicivirus, rotavirus, bocavirus, astrovirus and enterovirus. Through sequence-independent single primer amplification(SISPA) constructs a gene bank. Looks up the viral gene fragment in gene bank.</p><p><b>RESULTS</b>Obtaining corresponding viral acid sequence in six specimens.</p><p><b>CONCLUSION</b>This research can examine enterovirus and the virus which cause diarrhea, It make a foundation for further studies the viral cause of disease which the examination not yet discovered at present.</p>