Association of single nucleotide polymorphism of reduced folate carrier gene with susceptibility to acute leukemia / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 446-449, 2011.
Article
ي Zh
| WPRIM
| ID: wpr-326913
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the allele and genotype frequencies of reduced folate carrier gene (RFC) 80G/A polymorphism in Chinese patients with acute leukemia (AL) and healthy control children, and to provide clue for association between the single nucleotide polymorphism (SNP) of RFC and the occurrence of AL.</p><p><b>METHODS</b>Bone marrow samples from 98 childhood patients with AL and peripheral blood samples from 135 healthy children were obtained to prepare complementary DNAs (cDNAs). The cDNAs were analyzed for the polymorphisms in RFC 80G/A by reverse transcriptase-polymerase chain reaction-denaturing gradient gel electrophoresis (RT-PCR-DGGE) and direct sequencing.</p><p><b>RESULTS</b>The A allele frequencies of the AL patients and control children were 0.515 and 0.415, respectively (P< 0.05). Chi-square test confirmed a statistical significance of the association between RFC80 G/A and AL.</p><p><b>CONCLUSION</b>RFC 80AA or GA genotype may contribute to increasing the susceptibility to AL.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Base Sequence
/
Leukemia
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Acute Disease
/
Sequence Analysis, DNA
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Genetic Predisposition to Disease
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Polymorphism, Single Nucleotide
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Reduced Folate Carrier Protein
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Gene Frequency
/
Genetics
المحددات:
Adolescent
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
اللغة:
Zh
مجلة:
Chinese Journal of Medical Genetics
السنة:
2011
نوع:
Article