Cellular response to altered autophagy activity in human fibroblast cells overexpressing Ha-RasV12 / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of oncogenic Ras overexpression on autophagic activity in human fibroblast cells in vitro.</p><p><b>METHODS</b>BJ cells were transfected with H-RasV12 or control vector and treated with chloroquine, small interfering RNA (siRNA) for ATG7, or rapamycin. The cellular responses were analyzed by monitoring the parameters and biomarkers for cell growth, senescence and cell death.</p><p><b>RESULTS</b>In BJ cells overexpressing H-RasV12, chloroquine treatment resulted in more prominent cell senescence and a significantly increased cell death rate. Suppression of ATG7 mediated by siRNA also promoted cell senescence. Rapamycin treatment also caused an increased cell death rate but attenuated senescence in surviving cells. In control BJ cells, the cellular response to chloroquine included senescence and cell death, which occurred slowly. Rapamycin treatment and siRNA suppression of ATG7 had no obvious effect on control BJ cells.</p><p><b>CONCLUSION</b>Stable cellular overexpression of oncogenic Ras causes tightly controlled suppression of the autophagic activity of human fibroblast cells, and such changes produce significant effect on cell senescence and survival.</p>