Anticancer effect of triptolide-polyethylenimine-cyclodextrin in vitro / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 610-619, 2012.
Article
ي Zh
| WPRIM
| ID: wpr-336744
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a drug delivery system triptolide-polyethylenimine-cyclodextrin and to evaluate its anticancer activity in vitro.</p><p><b>METHODS</b>Triptolide was conjugated to polyethylenimine-cyclodextrin by N, N'-carbonyldiimidazole to form triptolide-polyethylenimine-cyclodextrin. (1)H-NMR, FT-IR and XRD were used to confirm its structure. The anticancer effect of the polymer was assessed by MTT assay, erasion trace test and hematoxylin-eosin staining. The potential to condense siRNA and to delivery siRNA into cytoplasm was demonstrated by gel retardation assay, zeta-potential determination and fluorescence staining.</p><p><b>RESULTS</b>Triptolide was successfully conjugated to polyethylenimine-cyclodextrin and the conjugation rate of triptolide was 10% (w/w). siRNA was effectively condensed by the polymer at the N/P ratio of 5, and its particle size was 300 ±15 nm and zeta potential was 8 ±2.5 mV. MTT assay, erasion trace test and hematoxylin-eosin staining revealed that triptolide-polyethylenimine-cyclodextrin had anticancer effect and low cytotoxicity to normal cells. The polymer was able to deliver siRNA to the cytoplasm effectively as demonstrated by fluorescence staining.</p><p><b>CONCLUSION</b>Triptolide-polyethylenimine-cyclodextrin is able to inhibit the growth and migration of cancer cells in vitro and to carry siRNA into cells effectively. It is potential to be used as a novel prodrug for co-delivery of gene and drug in cancer treatment.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Phenanthrenes
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Polyethyleneimine
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Polymers
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Drug Carriers
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Cyclodextrins
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Cell Line, Tumor
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Diterpenes
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Epoxy Compounds
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Nanoparticles
المحددات:
Humans
اللغة:
Zh
مجلة:
Journal of Zhejiang University. Medical sciences
السنة:
2012
نوع:
Article