Isolation, identification and structural characterization of secondary metabolites from amarine sponge-derived rare actinobacterium Dermacoccus sp. X4 / 生物工程学报
Chinese Journal of Biotechnology
; (12): 599-609, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-337438
المكتبة المسؤولة:
WPRO
ABSTRACT
We isolated and identified the symbiotic and adnascent microorganisms from an unidentified sponge collected from 10-meter-deep seawater of the Paracel Islands in China. A total of 16 strains were obtained and identified. Through bacteriostatic activity assay, one of the strains, Dermacoccus sp. X4, was found to effectively inhibit the growth of Staphylococcus aureus. Subsequently, its secondary metabolites were purified by silica gel partition, octadecylsilane (ODS) reverse phase, Sephadex™LH-20 size exclusion, and C18 reverse phase chromatography. Using liquid chromatography, mass spectrometry, and nuclear magnetic resonance, three of the purified compounds were structurally characterized to be one 3-(4-hydroxybenzyl) hexahydropyrrolo [1,2-a]pyrazine-1,4-dione and two indole acid glycerides. This is the first report about indole acid glyceride isolated from microbial secondary metabolites, enriching marine drug candidate resources.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Porifera
/
Seawater
/
Mass Spectrometry
/
Staphylococcus aureus
/
Actinomycetales
/
Magnetic Resonance Spectroscopy
/
Chemistry
/
China
/
Chromatography, Liquid
نوع الدراسة:
Diagnostic_studies
المحددات:
Animals
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
Zh
مجلة:
Chinese Journal of Biotechnology
السنة:
2016
نوع:
Article