Characterization of S gene of a strain of hantavirus isolated from Apodemus peninsulae in Heilongjiang Province / 病毒学报
Chinese Journal of Virology
; (6): 517-521, 2012.
Article
ي Zh
| WPRIM
| ID: wpr-340013
المكتبة المسؤولة:
WPRO
ABSTRACT
In order to study the molecular characterization of the hantavirus isolated from Apodemus peninsulae in Heilongjiang Province, the S gene of a new strain NA33 was amplified, sequenced and analyzed. The results showed that the complete nucleotide sequence of the S gene of NA33 strain was composed of 1 693 nucleotides with TA-rich. The S gene contained one ORF, starting at position 37 and ending at position 1 326, encoding the N protein of 429 amino acid residues, and in line with HTN-based coding. Sequence comparison of the S genes between NA33 and reference hantavirus strains showed that NA33 was more homologous to Amur-like viruses than to the Hantaan (HTN) viruses or the other hantaviruses. Phylogenetic analysis of the amino acid sequence of N proteins showed that NA33 was clustered into the group of Amur-like viruses and was more similar to Far East Russia and Jilin strains isolated from Apodemus peninsulae. The phylogenetic tree indicated a certain degree of host-dependent characteristics and geographical aggregation characteristics of hantanviruses. Furthermore, the amino acid sequence of N protein of NA33 had the conserved amino acid sites of Amur-like viruses. In conclusion, Apodemus peninsulae carried Amur-like viruses in Heilongjiang province and was an important infectious source of hemorrhagic fever with renal syndrome (HFRS).
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phylogeny
/
Rodent Diseases
/
Virology
/
Molecular Sequence Data
/
Chemistry
/
China
/
Viral Envelope Proteins
/
Classification
/
Orthohantavirus
/
Sequence Homology, Amino Acid
المحددات:
Animals
/
Humans
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
Zh
مجلة:
Chinese Journal of Virology
السنة:
2012
نوع:
Article