Effect of 2, 5-hexanedione on calcium homeostasis of motor neuron / 中华劳动卫生职业病杂志
Zhonghua laodong weisheng zhiyebing zazhi
; Zhonghua laodong weisheng zhiyebing zazhi;(12): 270-272, 2006.
Article
ي Zh
| WPRIM
| ID: wpr-342985
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of cytotoxic effect of 2, 5-hexanedione (2, 5-HD) on motor neuron.</p><p><b>METHODS</b>Vsc4.1 (a cell line from motor neuron) was incubated with a series concentration of 2, 5-HD. The cell viability, Ca(2+) Mg(2+) ATPase and Na(+)K(+) ATPase were detected. Laser scanning confocal microscope (LSCM) was used for detecting intracellular calcium level. The average calcium level in VSC4.1 was measured by flow cytometry.</p><p><b>RESULTS</b>The cell viability was decreased when Vsc4.1 cells were treated with 2, 5-HD at the dosage of 2.5, 5.0, 7.5, 10, 15 and 20 mmol/L for 24 hours. Compared with the control group the activity of Ca(2+) Mg(2+) ATPase was decreased to 70.02%, 77.44% and 47.47% respectively; the activity of Na(+)K(+) ATPase was decreased to 82.07%, 72.45% and 50.71%. The difference was significant. Intracellular free calcium of VSC4.1 cell was increased rapidly within 10 s and then recovered within 40 seconds when it was exposed to 33.5 mmol/L 2, 5-HD. An increase in intracellular calcium was observed when the VSC4.1 was treated with 33.5 mmol/L 2, 5-HD. The peak of intracellular calcium level occurred ten minutes later.</p><p><b>CONCLUSION</b>The disturbance of calcium homeostasis may be involved in the mechanisms of neurotoxicity of 2, 5-HD.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Cell Line
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Calcium
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Ca(2/) Mg(2/)-ATPase
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Sodium-Potassium-Exchanging ATPase
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Dose-Response Relationship, Drug
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Toxicity
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Hexanones
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Metabolism
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Motor Neurons
المحددات:
Animals
اللغة:
Zh
مجلة:
Zhonghua laodong weisheng zhiyebing zazhi
السنة:
2006
نوع:
Article