Influence of hepatocyte growth factor on biological characteristics of bone marrow-derived mesenchymal stem cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1044-1048, 2005.
Article
ي Zh
| WPRIM
| ID: wpr-343830
المكتبة المسؤولة:
WPRO
ABSTRACT
Hepatocyte growth factor (HGF) is one of major growth factors in the bone marrow microenvironments with which the proliferation, differentiation and migration of bone marrow-derived mesenchymal stem cells were closely contacted. However, its roles in the regulation of proliferation, differentiation and migration of bone marrow-derived mesenchymal stem cells remain unclear. This study was aimed to investigate the effect of HGF on biological characteristics of bone marrow-derived mesenchymal stem cells. Expression of c-Met, the receptor for HGF was detected by immunohistochemistry assay, cell proliferation was determined by MTT, activity of ALP was quantitatively assayed, cell migration and anoikis-induced MSC apoptosis were analyzed. The results showed that HGF not influenced the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Treatment of bone marrow-derived mesenchymal stem cells with recombinant human hepatocyte growth factor resulted in inhibition of anoikis-induced apoptosis. HGF significantly stimulated the migration of bone marrow-derived mesenchymal stem cells. Both PI-3 kinase and MAPK kinase were proved to be involved in HGF-induced migration. It is concluded that HGF/c-Met signal regulates the apoptosis and migration of bone marrow-derived mesenchymal stem cells.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Pharmacology
/
Bone Marrow Cells
/
Immunohistochemistry
/
Cell Movement
/
Cells, Cultured
/
Hepatocyte Growth Factor
/
Proto-Oncogene Proteins c-met
/
Anoikis
/
Cell Biology
/
Cell Proliferation
المحددات:
Humans
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2005
نوع:
Article