One-step multiplex RT-PCR for identifying common fusion transcripts in childhood acute lymphoblastic leukemia / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 332-336, 2015.
Article
ي Zh
| WPRIM
| ID: wpr-346153
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the efficiency of one-step multiplex RT-PCR for identifying four common fusion transcripts (TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL) in children with acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Total RNA was extracted from bone marrow samples of 76 children who were newly diagnosed with ALL between January 2003 and December 2010. These RNAs were analyzed for TEL/AML1, E2A/PBX1, MLL/AF4 and BCR/ABL by one-step multiplex RT-PCR or common nested-multiplex PCR. The PCR products were confirmed by DNA sequencing.</p><p><b>RESULTS</b>TEL/AML1 was found in 12 cases (the length of products was 298 bp in 9 cases and 259 bp in 3 cases), E2A/PBX1 was found in 3 cases (the length of products was 373 bp), BCR/ABL was found in 1 case (the length of products was 2 124 bp), and MLL/AF4 was found in 7 cases (the length of products was 427 bp in 1 case and 673 bp in 6 cases) using one-step multiplex RT-PCR combined with DNA sequencing. The results were consistent with those using common nested-multiplex PCR.</p><p><b>CONCLUSIONS</b>One-step multiplex RT-PCR may be another alternative for detection of common fusion transcripts in children with ALL.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Oncogene Proteins, Fusion
/
Fusion Proteins, bcr-abl
/
Sequence Analysis, DNA
/
Reverse Transcriptase Polymerase Chain Reaction
/
Myeloid-Lymphoid Leukemia Protein
/
Core Binding Factor Alpha 2 Subunit
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
Multiplex Polymerase Chain Reaction
/
Genetics
/
Methods
نوع الدراسة:
Prognostic_studies
المحددات:
Child
/
Child, preschool
/
Female
/
Humans
/
Infant
/
Male
اللغة:
Zh
مجلة:
Chinese Journal of Contemporary Pediatrics
السنة:
2015
نوع:
Article