Expression and purification of human amelogenin mature peptide in Escherichia coli / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 279-281, 2009.
Article
ي Zh
| WPRIM
| ID: wpr-346748
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish the expression and purification route for human amelogenin mature peptide in Escherichia coli and obtain the purified amelogenin (AMG) mature peptide.</p><p><b>METHODS</b>Recombined plasmid pGEX-4T-1-AMG was transformed to Escherichia coli BL21. After expression, AMG was purified with glutathione S-transferase fusion protein purification system (GSTrapFF) column.</p><p><b>RESULTS</b>Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting hybridization results showed that 45,000 GST-AMG fusing protein and 19,000 target AMG mature peptide were obtained successfully.</p><p><b>CONCLUSIONS</b>pGEX-4T-1-AMG-BL21 system is used successfully to express and purify human AMG mature peptide.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Peptides
/
Recombinant Fusion Proteins
/
Recombinant Proteins
/
Electrophoresis, Polyacrylamide Gel
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Escherichia coli
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Amelogenin
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Genetics
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Glutathione Transferase
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Metabolism
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Stomatology
السنة:
2009
نوع:
Article