Expression and purification of human amelogenin mature peptide in Escherichia coli / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the expression and purification route for human amelogenin mature peptide in Escherichia coli and obtain the purified amelogenin (AMG) mature peptide.</p><p><b>METHODS</b>Recombined plasmid pGEX-4T-1-AMG was transformed to Escherichia coli BL21. After expression, AMG was purified with glutathione S-transferase fusion protein purification system (GSTrapFF) column.</p><p><b>RESULTS</b>Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting hybridization results showed that 45,000 GST-AMG fusing protein and 19,000 target AMG mature peptide were obtained successfully.</p><p><b>CONCLUSIONS</b>pGEX-4T-1-AMG-BL21 system is used successfully to express and purify human AMG mature peptide.</p>