Pharmacokinetic study on three main ingredients of refined coronary cataplasm / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3641-3646, 2012.
Article
ي Zh
| WPRIM
| ID: wpr-346891
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a LC-MS/MS method for determining the concentration of tanshinone IIA, salvianolic acid B and paeoniflorin of refined coronary cataplasm in rabbit plasma, in order to determine the concentration of the three main ingredients in blood after transdermal administration and calculate their pharmacokinetic parameters.</p><p><b>METHOD</b>Rabbits were given refined coronary cataplasm on the basis of 15 g x kg(-1) by transdermal administration to detect the plasma concentration of the three main ingredients using LC-MS/MS. Winnonlin software was used to calculate their major pharmacokinetic parameters.</p><p><b>RESULT</b>Tanshinone IIA, salvianolic acid B and paeoniflorin showed good linearity (r>0.999) at 1-100, 50-1 000, 10-1 000 microg x L(-1) respectively in plasma, with average recovery rate of 96.57%, 91.90%, 95.93%, respectively. The RSD within day were less than 15%. After transdermal administration of refined coronary cataplasm in rabbits, the main pharmacokinetic parameters of tanshinone IIA, salvianolic acid B or paeoniflorin were as follows: Cmax (20.85 +/- 12.68), (636.25 +/- 386.91), (787.80 +/- 395.64) microg x L(-1); Tmax (0.49 +/- 0.28), (0.44 +/- 0.27), (0.46 +/- 0.30) h.</p><p><b>CONCLUSION</b>The LC-MS/MS method is highly selective and sensitive to determine the concentration of samples in rabbit plasma. The pharmacokinetic characteristics of tanshinone IIA, salvianolic acid B and paeoniflorin are suitable to assess the percutaneous absorption of refined coronary cataplasm.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Benzoates
/
Benzofurans
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Bridged-Ring Compounds
/
Drugs, Chinese Herbal
/
Pharmacokinetics
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Chromatography, Liquid
/
Coronary Disease
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Monoterpenes
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Abietanes
/
Drug Therapy
المحددات:
Animals
/
Humans
/
Male
اللغة:
Zh
مجلة:
China Journal of Chinese Materia Medica
السنة:
2012
نوع:
Article