Expression and gene polymorphisms of B cell activating factor in patients with idiopathic thrombocytopenic purpura / 中华血液学杂志
Chinese Journal of Hematology
; (12): 586-589, 2010.
Article
ي Zh
| WPRIM
| ID: wpr-353612
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the polymorphisms of B cell activating factor (BAFF) gene and the plasma levels of BAFF in patients with idiopathic thrombocytopenic purpura (ITP), and to investigate their roles in the pathogenesis of ITP.</p><p><b>METHODS</b>Alleles specific polymerase chain reaction (AS-PCR) and agarose gel electrophoresis were used to identify polymorphisms -871C/T of BAFF promotor in 133 ITP patients and 117 healthy controls. The plasma levels of BAFF were assayed by ELISA.</p><p><b>RESULTS</b>In ITP group, the frequency of C/C, C/T and T/T was 33.1%, 42.1% and 24.8%, respectively, the corresponding frequency in control group was 55.6%, 33.3% and 11.1%, respectively. The allele frequency of T in ITP and control groups was 45.9% and 27.4%, respectively. There was a significant difference in the BAFF -871C/T genotypic frequency between the ITP and control groups (P < 0.05). BAFF antigen in untreated ITP, treated patients and controls was 875.86 pg/ml, 502.59 pg/ml and 736.88 pg/ml, respectively, being also a significant difference among the three groups (P < 0.05). BAFF antigen in homozygous T/T was higher than that in homozygous C/C and heterozygous C/T, but the difference was not statistically significant (P > 0.05).</p><p><b>CONCLUSIONS</b>Over expression of BAFF may be a risk factor for ITP patients. There is a correlation of the BAFF promotor polymorphism -871C/T with ITP, but the polymorphism does not affect the expression of BAFF.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Polymorphism, Genetic
/
Interleukin-4
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Purpura, Thrombocytopenic, Idiopathic
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Allergy and Immunology
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B-Cell Activating Factor
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Gene Frequency
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Genetics
نوع الدراسة:
Prognostic_studies
/
Risk_factors_studies
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Hematology
السنة:
2010
نوع:
Article