Genome amplification and sequence analysis of human bocavirus 2 / 病毒学报
Chinese Journal of Virology
; (6): 257-262, 2014.
Article
ي Zh
| WPRIM
| ID: wpr-356606
المكتبة المسؤولة:
WPRO
ABSTRACT
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phylogeny
/
Virology
/
RNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Chemistry
/
Gene Amplification
/
Classification
/
Genome, Viral
/
Parvoviridae Infections
المحددات:
Humans
اللغة:
Zh
مجلة:
Chinese Journal of Virology
السنة:
2014
نوع:
Article