Expression of N-methyl-D-aspartate receptor 1 in acute intoxicated encephalopathy induced by 1,2-dichloroethane / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 65-68, 2007.
Article
ي Zh
| WPRIM
| ID: wpr-357605
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of N-methyl-D-aspartate receptor 1 in acute intoxicated encephalopathy induced by 1,2- dichloroethane(1,2-DCE).</p><p><b>METHODS</b>Forty-two Sprague-Dawley rats, which had been randomly divided into 1 control, 3 exposure and 3 after-exposure observation groups were exposed to 1,2-DCE for 12 hr by continual static inhalation except control group. Dosage of exposure groups was 5.0, 10.0, 20.0 g/m(3) on sequence. That of after-exposure observation groups was 10.0 g/m(3). Rats of after-exposure observation groups were observed continually for 2,4,6 hr after exposure. The expression of N-methyl-D-aspartate receptor-1 (NMDAR1) was detected by immunohistochemical method.</p><p><b>RESULTS</b>NMDAR1 stained neurons were mainly distributed at cerebral cortex and hippocampus. Compared with that of control group, the percentages of positive cells of NMDAR1 increased evidently at 10.0, 20.0 g/m(3) groups (P < 0.05). They were (18.33 +/- 1.86)%, (64.17 +/- 2.86)% at cerebral cortex, (15.5 +/- 1.87)%, (47.83 +/- 2.16)% at hippocampus. The percentages were also elevated obviously in 2, 4, 6 h after-exposure observation groups. They were (39.07 +/- 3.01)% (70.17 +/- 2.93)% (39.83 +/- 2.32)% at cerebral cortex, (16.30 +/- 1.03)% (19.80 +/- 1.17)% (16.50 +/- 1.05)% at hippocampus; Compared with that of 10.0 g/m(3) group, the percentages increased significantly only in 4 hr group at hippocampus.</p><p><b>CONCLUSION</b>The overactivation of NMDAR1 is the main route by which excitatory amino acids chose to join the development of acute intoxicated encephalopathy induced by 1,2-DCE.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Rats, Sprague-Dawley
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Receptors, N-Methyl-D-Aspartate
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Neurotoxicity Syndromes
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Ethylene Dichlorides
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Toxicity
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Hippocampus
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Metabolism
المحددات:
Animals
اللغة:
Zh
مجلة:
Chinese Journal of Industrial Hygiene and Occupational Diseases
السنة:
2007
نوع:
Article