Bacterial entombment by intratubular mineralization following orthograde mineral trioxide aggregate obturation: a scanning electron microscopy study / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 227-232, 2014.
Article
ي En
| WPRIM
| ID: wpr-358133
المكتبة المسؤولة:
WPRO
ABSTRACT
The time domain entombment of bacteria by intratubular mineralization following orthograde canal obturation with mineral trioxide aggregate (MTA) was studied by scanning electron microscopy (SEM). Single-rooted human premolars (n=60) were instrumented to an apical size #50/0.06 using ProFile and treated as follows Group 1 (n=10) was filled with phosphate buffered saline (PBS); Group 2 (n=10) was incubated with Enterococcus faecalis for 3 weeks, and then filled with PBS; Group 3 (n=20) was obturated orthograde with a paste of OrthoMTA (BioMTA, Seoul, Korea) and PBS; and Group 4 (n=20) was incubated with E. faecalis for 3 weeks and then obturated with OrthoMTA-PBS paste. Following their treatments, the coronal openings were sealed with PBS-soaked cotton and intermediate restorative material (IRM), and the roots were then stored in PBS for 1, 2, 4, 8 or 16 weeks. After each incubation period, the roots were split and their dentin/MTA interfaces examined in both longitudinal and horizontal directions by SEM. There appeared to be an increase in intratubular mineralization over time in the OrthoMTA-filled roots (Groups 3 and 4). Furthermore, there was a gradual entombment of bacteria within the dentinal tubules in the E. faecalis inoculated MTA-filled roots (Group 4). Therefore, the orthograde obturation of root canals with OrthoMTA mixed with PBS may create a favorable environment for bacterial entombment by intratubular mineralization.
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Oxides
/
Physiology
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Root Canal Filling Materials
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Root Canal Obturation
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Time Factors
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Zinc Oxide-Eugenol Cement
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Calcification, Physiologic
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Microscopy, Electron, Scanning
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Enterococcus faecalis
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Silicates
المحددات:
Humans
اللغة:
En
مجلة:
International Journal of Oral Science
السنة:
2014
نوع:
Article