Fluoxetine Up-Regulates Bcl-xL Expression in Rat C6 Glioma Cells
Psychiatry Investigation
; : 161-168, 2011.
Article
ي En
| WPRIM
| ID: wpr-35972
المكتبة المسؤولة:
WPRO
ABSTRACT
OBJECTIVE: To analyze both differentially expressed genes and the Bcl-xL protein expression after acute and chronic treatment with fluoxetine in rat C6 glioma cells. METHODS: C6 glioma cells were cultured for 24 h or 72 h after treatment with 10 microM fluoxetine, and gene expression patterns were observed using microarray and qRT-PCR. Then, cells were cultured for 6 h, 24 h, 72 h or 96 h after treatment with 10 microM fluoxetine, and the expression of Bcl-xL protein was measured using western blot. RESULTS: As determined by microarray, treatment with fluoxetine for 24 h up-regulated 33 genes (including Bcl-xL and NCAM140) and down-regulated 7 genes (including cyclin G-associated kinase). Treatment with fluoxetine for 72 h up-regulated 53 genes (including Gsalpha and Bcl-xL) and down-regulated 77 genes (including Galphai2 and annexin V). Based on the qRT-PCR results, there was an increase in Gsalpha mRNA and a decrease in Galphai2 mRNA at 72 h in fluoxetine-treated cells as compared to control, a result that was consistent with microarray. We also observed an increase in Bcl-xL mRNA (both at 24 h and at 72 h) in fluoxetine-treated cells as compared to control, demonstrating a tendency to increase gradually. Bcl-xL protein expression increased as the duration of fluoxetine treatment increased. CONCLUSION: These results suggest that chronic treatment with fluoxetine not only initiates the cAMP pathway through inducing Gsalpha expression but also induces Bcl-xL expression, thus inhibiting apoptosis.
Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
RNA, Messenger
/
Gene Expression
/
Fluoxetine
/
Apoptosis
/
Cyclins
/
Bcl-X Protein
/
Glioma
المحددات:
Animals
اللغة:
En
مجلة:
Psychiatry Investigation
السنة:
2011
نوع:
Article