Effect of MicroRNA-146a on Differentiation Potential of Human Bone Marrow Mesenchymal Stem Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 596-601, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-360041
المكتبة المسؤولة:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of MicroRNA-146a (miR-146a) on the ability of BM-MSC to differentiate into adipocytes and osteoblasts.</p><p><b>METHODS</b>BM-MSC were isolated from the bone marrow of healthy donors. The differentiation of BM-MSC into adipocytes and osteoblasts cells were done in vitro. After transfection with miR-146a inhibitor or mimics, the expression of miR-146a in BM-MSC was detected by real time quantitative PCR. The effect of MicroRNA-146a on the differentiation potential of BM-MSC was evaluated after transfection.</p><p><b>RESULTS</b>BM-MSC possessed the ability to differentiate into adipocytes and osteoblasts cells when cultured in the induction medium. The expression of miR-146a was correspondingly down-regulated and up-regulated in BM-MSC after transfection. Compared with the control group, the expression of miR-146a was down-regulated (P < 0.01) after transfection with miR-146a inhibitor, while after transfection with miR-146a mimics it was significantly up-regulated. This study proved that the transfection with miR-146a inhibitor can inhibit BM-MSC differentiate into adipocytes (P < 0.01), while transfection with miR-146a mimics can promote differentiation of BM-MSC into adipocytes (P < 0.01). No effect of miR-146a inhibitor or miR-146a mimics on osteogenic differentiation of BM-MSC was observed (P > 0.05).</p><p><b>CONCLUSION</b>BM-MSC possess the ability to differentiate into adipocytes and osteoblasts. The miR-146a can promote BM-MSC to differentiate into adipocytes.</p>
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Osteoblasts
/
Osteogenesis
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Bone Marrow Cells
/
Transfection
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Cell Differentiation
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Cells, Cultured
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Adipocytes
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Cell Biology
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MicroRNAs
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Mesenchymal Stem Cells
المحددات:
Humans
اللغة:
Zh
مجلة:
Journal of Experimental Hematology
السنة:
2016
نوع:
Article