Comparison of MALDI-TOF MS, Housekeeping Gene Sequencing, and 16S rRNA Gene Sequencing for Identification of Aeromonas Clinical Isolates
Yonsei Medical Journal
; : 550-555, 2015.
Article
ي En
| WPRIM
| ID: wpr-38895
المكتبة المسؤولة:
WPRO
ABSTRACT
PURPOSE:
The genus Aeromonas is a pathogen that is well known to cause severe clinical illnesses, ranging from gastroenteritis to sepsis. Accurate identification of A. hydrophila, A. caviae, and A. veronii is important for the care of patients. However, species identification remains difficult using conventional methods. The aim of this study was to compare the accuracy of different methods of identifying Aeromonas at the species level a biochemical method, matrix-assisted laser desorption ionization mass spectrometry-time of flight (MALDI-TOF MS), 16S rRNA sequencing, and housekeeping gene sequencing (gyrB, rpoB). MATERIALS ANDMETHODS:
We analyzed 65 Aeromonas isolates recovered from patients at a university hospital in Korea between 1996 and 2012. The isolates were recovered from frozen states and tested using the following fourmethods:
a conventional biochemical method, 16S rRNA sequencing, housekeeping gene sequencing with phylogenetic analysis, and MALDI-TOF MS.RESULTS:
The conventional biochemical method and 16S rRNA sequencing identified Aeromonas at the genus level very accurately, although species level identification was unsatisfactory. MALDI-TOF MS system correctly identified 60 (92.3%) isolates at the species level and an additional four (6.2%) at the genus level. Overall, housekeeping gene sequencing with phylogenetic analysis was found to be the most accurate in identifying Aeromonas at the species level.CONCLUSION:
The most accurate method of identification of Aeromonas to species level is by housekeeping gene sequencing, although high cost and technical difficulty hinder its usage in clinical settings. An easy-to-use identification method is needed for clinical laboratories, for which MALDI-TOF MS could be a strong candidate.Key words
النص الكامل:
1
الفهرس:
WPRIM
الموضوع الرئيسي:
Phylogeny
/
DNA, Bacterial
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RNA, Ribosomal, 16S
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Sensitivity and Specificity
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Sequence Analysis, DNA
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Aeromonas
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Genes, Essential
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Republic of Korea
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Molecular Typing
نوع الدراسة:
Diagnostic_studies
المحددات:
Humans
البلد/الأقليم حسب الموضوع:
Asia
اللغة:
En
مجلة:
Yonsei Medical Journal
السنة:
2015
نوع:
Article