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MODULATORY EFFECT OF CAFFEINE ON GABA-ACTIVATED CURRENT FROM ACUTELY ISOLATED RAT DORSAL ROOT GANGLION NEURONS / 神经解剖学杂志
Chinese Journal of Neuroanatomy ; (6): 251-255, 2007.
Article ي Zh | WPRIM | ID: wpr-407840
المكتبة المسؤولة: WPRO
ABSTRACT
Whole-cell patch clamp technique was performed on acutely isolated rat dorsal root ganglion (DRG) neurons to investigate the modulatory effect of caffeine on γ-aminobutyric acid (GABA)-activated currents (IGABA). The results showed that the majority of the neurons examined (97.4%, 113/116) were sensitive to GABA. 1-1000 μmol/L GABA activated a concentration-dependent inward current which manifested obvious desensitization. After the neurons were treated with caffeine (0.01-100 μmol/L) prior to the application of GABA (100 μmol/L) for 30 s, GABA-activated membrane currents were obviously inhibited. Caffeine shifted the GABA dose-response curve downward and decreased the maximum response to 57% without changing Kd value. These results indicate that the inhibitory effect is non-competitive. Theophylline showed a similar and stronger inhibitory effect on IGABA. The pretreatment with caffeine (10 μmol/L) inhibited IGABA, which was potentized by diazepam (1 μmol/L). Intracellular application of H-8 almost completely abolished the inhibitory effect of caffeine on IGABA. The present results suggest that caffeine may be able to antagonize the effect of presynaptic inhibition of GABA in primary afferent.
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النص الكامل: 1 الفهرس: WPRIM اللغة: Zh مجلة: Chinese Journal of Neuroanatomy السنة: 2007 نوع: Article
النص الكامل: 1 الفهرس: WPRIM اللغة: Zh مجلة: Chinese Journal of Neuroanatomy السنة: 2007 نوع: Article