Determination of Ethyl Pyruvate by GC / 中国药师
China Pharmacist
; (12): 1021-1023, 2016.
Article
ي Zh
| WPRIM
| ID: wpr-494033
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective:
To establish a method for the determination of ethyl pyruvate by GC.Methods:
A Varian CP7502 capillary column(25 m × 0. 25 mm,0. 25 μm)was used. The carrier gas was nitrogen with the flow rate of 30 ml·min-1 ,the gas was hydrogen with the flow rate of 40 ml·min -1 and the oxidant gas was air with the flow rate of 400 ml·min-1 . The detector was an FID and the inlet temperature was 210℃ . The temperature program was as followsthe initial column temperature was 40℃, and then risen to 200℃ with a rate of 15 ℃·min -1 . The split ratio was 100 ∶1 and the injection volume was 1μl.Results:
Ethyl pyruvate had a good linear relationship within the range of 0. 025 6- 8. 192 8 mg·ml -1 ,and r was 0. 999 8. The average recovery was99. 99% and RSD was 1. 38%(n = 9).Conclusion:
The method is accurate,simple and rapid with good stability and high sensitivity,which can be used to determine the content of ethyl pyruvate.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
China Pharmacist
السنة:
2016
نوع:
Article