Quantitative analysis of γ-oryzanol content in cold pressed rice bran oil by TLC-image analysis method
Asian Pacific Journal of Tropical Biomedicine
; (12): 119-123, 2014.
Article
ي Zh
| WPRIM
| ID: wpr-500492
المكتبة المسؤولة:
WPRO
ABSTRACT
Objective:
To develop and validate an image analysis method for quantitative analysis ofγ-oryzanol in cold pressed rice bran oil.Methods:
TLC-densitometric and TLC-image analysis methods were developed, validated, and used for quantitative analysis of γ-oryzanol in cold pressed rice bran oil. The results obtained by these two different quantification methods were compared by paired t-test.Results:
Both assays provided good linearity, accuracy, reproducibility and selectivity for determination of γ-oryzanol.Conclusions:
The TLC-densitometric and TLC-image analysis methods provided a similar reproducibility, accuracy and selectivity for the quantitative determination of γ-oryzanol in cold pressed rice bran oil. A statistical comparison of the quantitative determinations of γ-oryzanol in samples did not show any statistically significant difference between TLC-densitometric and TLC-image analysis methods. As both methods were found to be equal, they therefore can be used for the determination of γ-oryzanol in cold pressed rice bran oil.
النص الكامل:
1
الفهرس:
WPRIM
اللغة:
Zh
مجلة:
Asian Pacific Journal of Tropical Biomedicine
السنة:
2014
نوع:
Article