Role of hypoxia- inducible factor-1 in cardioprotection of hypoxic preconditioning / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12)1986.
Article
ي Zh
| WPRIM
| ID: wpr-522299
المكتبة المسؤولة:
WPRO
ABSTRACT
AIM: To investigate the effects of hypoxia-inducible factor-1 (HIF-1) on cardioprotection of hypoxic preconditioning (HPC) and its mechanisms. METHODS: In the model of hypoxia/reoxygenation (H/R) of cardiomyocytes from neonatal Spargue-Dawley rats, delayed cardioprotective effects of HPC on lethal H/R were observed. Whole cell extracts were prepared for determining activities of extracellular signal-regulated protein kinases (ERKs) and HIF-1? phosphorylation. RESULTS: HPC significantly promoted survival and membrane integrity of cardiomyocytes subjected to sustained H/R. SDS-PAGE mobility shift experiments showed increased phophorylation level of HIF-1? in hypoxic preconditioned cardiomyocytes. ERK1/2 were activated at 10 min after HPC and returned to the control level at 60 min. CONCLUSION: HPC protects neonatal cardiomyocytes from H/B injury. HIF-1? phosphorylation induced by ERKs might contribute to the cardioprotection of HPC.
النص الكامل:
1
الفهرس:
WPRIM
نوع الدراسة:
Prognostic_studies
اللغة:
Zh
مجلة:
Chinese Journal of Pathophysiology
السنة:
1986
نوع:
Article