High density lipoprotein subclasses and activities of protein kinase C / 中国病理生理杂志

ABSTRACT

AIM: To investigate the relationship between high density lipoprotein (HDL) subclasses and the binding activities of hepatic cell and extra-hepatic cell HDL receptor and intracellular protein kinase C (PKC). METHODS: Using purified pre-?_1 HDL and apoE-deficent HDL_3 to react with human smooth muscle cells (SMC) and HepG2 cells, then the activities of pre-?_1HDL and apoE-deficent HDL_3 binding to SMC and HepG2 cells, and the effects of this two HDL subclasses on PKC activities in SMC and HepG2 cells were observed . RESULTS: The results showed that the value of Kd of pre-?_1HDL binding to SMC HDL receptor was not only significantly lower than that of apoE-deficient HDL_3 (P